JASCO | Fluorescence Anisotropy and Polarization Measurement
When used Fluorescence Anisotropy and Polaization Measurment ?
When used Fluorescence Anisotropy and Polaization Measurment ?
→Fluorescence anisotropy occurs when a fluorophore emits different intensities of light dependent on the polarization angle of the incident light.
Anisotropy can be used to probe the structural flexibility of a fluorophore, which cannot be obtained by fluorescence spectroscopy alone.
The FDP-837 is used to automatically measure fluorescence anisotropy and the degree of polarization using two wide spectral range polarizers mounted in the excitation and emission paths in the sample compartment.
[ Wavelength range: 220-700 nm ]
The two manually adjustable polarizers can be used to measure anisotropy in the UV-Visible (FDP-223) and Visible only (FDP-423) ranges.
For solid-state and viscous samples with polarization characteristics, the depolarization plate is a filter that can be used to depolarize the excitation light.