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Flavonoid–Sesquiterpenoid Hybrids from the Leaves of Syzygium simile and Their Anti-Lipid Droplet Accumulation Activities
2025 / Journal of Natural ProductsMetabolic-associated fatty liver disease (MAFLD) represents a spectrum of hepatic disorders characterized by excessive lipid accumulation in the liver with a global prevalence rate of 30%. Despite their increasing prevalence, current therapeutic interventions remain suboptimal, constrained by substantial adverse effects and prohibitive treatment costs. Through an a...
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Applying Isothermal Titration Calorimetry and Saturation Transfer Difference-NMR to Study the Mode of Interaction of Flavan-3-ols with α-Amylase to Understand Their Impact on Starch Hydrolysis
2025 / Journal of Agricultural and FoodFor flavan-3-ols, significant effects to prevent the development of diabetes mellitus are postulated. Inter alia, this is attributed to inhibitory effects on the intestinal α-amylase, in particular for high-molecular-weight procyanidins. In order to gain a deeper insight into the mode of interaction and the resulting α-amylase inhibition, the interactio...
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Insights of the trypsin inhibitory activity and ultrasound effect of ovomucoid based on molecular docking and spectroscopic
2024 / Food, Nutrition and HealthOvomucoid (OVM) is the most abundant trypsin inhibitor in egg white, considered as one of the anti-nutritional factors in egg processing. In this study, the molecular mechanism of the interaction between OVM and trypsin was explored. Besides, the effect of food processing methods on the activity of OVM and structural change of OVM was analyzed. The results showed...
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Partial efficacy of Yuzu (Citrus junos) pectin side chains in elongating the jejunal villus in protein-deficient malnourished mice
2024 / Journal of Functional FoodsPectin, a soluble fiber, can induce elongation of the intestinal villi. However, the relationship between pectin-induced villus elongation and nutrient absorption remains unclear. Therefore, we investigated whether pectin could mitigate malnutrition by enhancing nutrient absorption. We specifically utilized Yuzu pectin side chains, which were chosen for their poten...
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Dereplication of Bioactive Agave Saponin Fractions: The Hidden Saponins
2024 / Journal of Agricultural and Food ChemistryThe good phytotoxicity and selectivity against weeds versus tomato or cress make saponin-rich fractions from Agave macroacantha, A. colorata, A. parryi, and A. parrasana attractive candidates as bioherbicides. The saponin contents have only previously been reported for A. macroacantha, and as a consequence, simultaneous dereplication has been performed on saponin-r...
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Essential oil vapour inclusion in protective atmosphere packaging for shelf-life management of fisheries
2024 / International Journal of Food Science & TechnologyFish is well known for its perishable nature. Essential oils in food packaging seem to be safe and reliable both in terms of safety for the consumer and in terms of improving the shelf life of the product. This study aimed to evaluate the effects of two different essential oil vapours (one with rosemary and the other one with lemon extract) on three different typ...
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Identification of a Novel Peptide with Alcohol Dehydrogenase Activating Ability from Ethanol-Induced Lactococcus lactis: A Combined In Silico Prediction and In Vivo Validation
2024 / Journal of Agricultural and Food ChemistryAlcohol dehydrogenase (ADH) is a crucial rate-limiting enzyme in alcohol metabolism. Our previous research found that ethanol-induced intracellular extracts of Lactococcus lactis (L. lactis) could enhance alcohol metabolism in mice, but the responsible compounds remain unidentified. The study aimed to screen potential ADH-activating peptides from ethanol-induced L....
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De Novo Access to BODIPY C-Glycosides as Linker-Free Nonsymmetrical BODIPY-Carbohydrate Conjugates
2024 / The Journal of Organic ChemistryRecent years have witnessed an increasing interest in the synthesis and study of BODIPY-glycoconjugates. Most of the described synthetic methods toward these derivatives involve postfunctional modifications of the BODIPY core followed by the covalent attachment of the fluorophore and the carbohydrate through a “connector”. Conversely, few de novo synthe...
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Phytotoxic and Antimicrobial Terrein Derivatives and Butenolides Isolated from the Endophytic Fungus Aspergillus terreus HT5
2023 / Journal of Agricultural and Food ChemistryTwo new terrein derivatives, aspergilethers A and B (1 and 2), two known analogues (3 and 4), and three known butenolides (5–7) were isolated from the endophyte Aspergillus terreus HT5. Their structures were determined by spectroscopic analysis and ECD and NMR calculations. Interestingly, 1 and 2 had unpresented medium aliphatic side chains in terrein derivat...
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Hydrolysis of Gluten-Derived Celiac Disease-Triggering Peptides across a Broad pH Range by RmuAP1: A Novel Aspartic Peptidase Isolated from Rhodotorula mucilaginosa
2023 / Journal of Agricultural and Food ChemistryAn aspartate peptidase with proteolytic activity toward gluten was identified from an isolated red yeast Rhodotorula mucilaginosa strain. This peptidase consists of 425 amino acids, comprising an N-terminal signal peptide, a propeptide, and a C-terminal catalytic domain. The catalytic domain, termed RmuAP1CD, could be secreted by the recombinant oleaginous yeast Ya...
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Characterization of Oyster Protein Hydrolysate–Iron Complexes and their In Vivo Protective Effects against Iron Deficiency-Induced Symptoms in Mice
2023 / Journal of Agricultural and Food ChemistryIron is one of the trace mineral elements, and iron deficiency is a common phenomenon that negatively influences human health. Food-derived iron supplements were considered excellent candidates for improving this syndrome. In this work, oyster-protein hydrolysates (OPH) and ferrous chloride successfully formed the OPH–Fe complex (6 mg/mL, 40 °C, 30 min), ...
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Residue Effect-Guided Design: Engineering of S. Solfataricus β-Glycosidase to Enhance Its Thermostability and Bioproduction of Ginsenoside Compound K
2023 / Journal of Agricultural and Food Chemistryβ-Glycosidase from Sulfolobus solfataricus (SS-BGL) is a highly effective biocatalyst for the synthesis of compound K (CK) from glycosylated protopanaxadiol ginsenosides. In order to improve the thermal stability of SS-BGL, molecular dynamics simulations were used to determine the residue-level binding energetics of ginsenoside Rd in the SS-BGL-Rd docked compl...
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Oligomerization Mechanisms of Tea Catechins Involved in the Production of Black Tea Thearubigins
2024 / Journal of Agricultural and Food ChemistryThearubigins (TRs) are chemically ill-defined black tea pigments composed of numerous catechin oxidation products. TRs contain oligomeric components; however, the oligomerization mechanisms are poorly understood. The comparison of the 13C nuclear magnetic resonance (NMR) spectra of TRs with different molecular sizes suggested the participation of A-ring methine car...
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Beverages,Dairy products,Iron,Membranes,Peptides and proteinsShow Less
2023 / Journal of Agricultural and Food ChemistryBovine lactoferrin (bLF) is widely known as an iron-binding glycoprotein from the transferrin family. The bLF molecule exhibits a broad spectrum of biological activity, including iron delivery, antimicrobial, antiviral, immunomodulatory, antioxidant, antitumor, and prebiotic functions, thereby making it one of the most valuable representatives for biomedical applic...
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Cocultures of Enterococcus faecium and Aeromonas veronii Induce the Secretion of Bacteriocin-like Substances against Aeromonas
2023 / Journal of Agricultural and Food ChemistryLactic acid bacteria (LAB) were screened from Lutjanus russellii (red sea bass), and their antimicrobial activities were evaluated against two Aeromonas species isolated from the Nile tilapia, namely, Aeromonas veronii (AV) and Aeromonas jandaei (AJ). Three LAB isolates, Enterococcus faecium MU8 (EF_8), Enterococcus faecalis MU2 (EFL_2), and E. faecalis MU9 (EFL_9)...
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